polyclonal rabbit anti gr primary antibody  (Danaher Inc)

Journal: Endocrinology

Article Title: Glucocorticoid receptor signaling is essential for mesoderm formation and muscle development in zebrafish.

doi: 10.1210/en.2011-1559

Figure Lengend Snippet: FIG. 1. GR gene and protein expression in WT embryos. GR mRNA and protein exhibit dynamic spatial expression during early embryogenesis. A, Spatial GR mRNA abundance in WT embryos at 16-cell (I), 4-hpf (II), 6-hpf (III), 8-hpf (IV), 10-hpf (V), 12-hpf (VI), 24-hpf (VII), and-48 hpf (VIII) stages (n 10–15 embryos at each stage). Sense strand hybridizations at 24 hpf (IX) and 48 hpf (X) display probe specificity. B, Spatial GR protein expression in WT embryos (n 10–12 embryos) at 24 hpf (I), 36 hpf (II), and 48 hpf (III). Fluorescent images (left) show GR protein distribution detected by specific antibody (polyclonal rabbit antizebrafish GR) binding, brightfield images (middle) display whole embryo shape, and negative control (Neg. Ctrl.) images (right) show specificity of fluorescence in embryo tissue.

Article Snippet: The proteins were transferred onto a nitrocellulose membrane and were probed with the rabbit antizebrafish GR primary antibody (1: 750 dilution) and goat antirabbit horseradish peroxidase-conjugated secondary antibody (1:3000 dilution; Bio-Rad, Hercules, CA) for 1 h each at room temperature with gentle rocking.

Techniques: Expressing, Binding Assay, Negative Control, Fluorescence