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polyclonal rabbit anti gr primary antibody  (Danaher Inc)


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    Danaher Inc polyclonal rabbit anti gr primary antibody
    Polyclonal Rabbit Anti Gr Primary Antibody, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/polyclonal+rabbit+anti+gr+primary+antibody/pmc11279577-122-12-19
    Average 86 stars, based on 1 article reviews
    polyclonal rabbit anti gr primary antibody - by Bioz Stars, 2026-09
    86/100 stars

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    Related Articles

    Incubation:

    Article Title: Juvenile/Peripubertal Exposure to Omega-3 and Environmental Enrichment Differentially Affects CORT Secretion and Adulthood Stress Coping, Sociability, and CA3 Glucocorticoid Receptor Expression in Male and Female Rats
    Article Snippet: .. Tissues were rinsed prior to overnight incubation at 4 °C with the polyclonal rabbit anti-GR primary antibody (1:500; ab3578; Abcam, Toronto, ON, Canada) mixed in blocking solution. .. The following day, brain sections were washed in PBS (3 × 5 min) and incubated with Alexa 594-conjugated donkey anti-rabbit secondary antibody (1:1000; A-21207; Invitrogen Canada Inc., Burlington, ON, Canada) at RT in the dark for 1 h. Sections were rinsed with PBS (3 × 5 min) and immersed in Hoechst adenine–thymine-binding dye (1:20,000; Hoechst 33342, Invitrogen Canada Inc.) for 5 min at RT.

    Blocking Assay:

    Article Title: Juvenile/Peripubertal Exposure to Omega-3 and Environmental Enrichment Differentially Affects CORT Secretion and Adulthood Stress Coping, Sociability, and CA3 Glucocorticoid Receptor Expression in Male and Female Rats
    Article Snippet: .. Tissues were rinsed prior to overnight incubation at 4 °C with the polyclonal rabbit anti-GR primary antibody (1:500; ab3578; Abcam, Toronto, ON, Canada) mixed in blocking solution. .. The following day, brain sections were washed in PBS (3 × 5 min) and incubated with Alexa 594-conjugated donkey anti-rabbit secondary antibody (1:1000; A-21207; Invitrogen Canada Inc., Burlington, ON, Canada) at RT in the dark for 1 h. Sections were rinsed with PBS (3 × 5 min) and immersed in Hoechst adenine–thymine-binding dye (1:20,000; Hoechst 33342, Invitrogen Canada Inc.) for 5 min at RT.



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    FIG. 1. GR gene and protein expression in WT embryos. GR mRNA and protein exhibit dynamic spatial expression during early embryogenesis. A, Spatial GR mRNA abundance in WT embryos at 16-cell (I), 4-hpf (II), 6-hpf (III), 8-hpf (IV), 10-hpf (V), 12-hpf (VI), 24-hpf (VII), and-48 hpf (VIII) stages (n 10–15 embryos at each stage). Sense strand hybridizations at 24 hpf (IX) and 48 hpf (X) display probe specificity. B, Spatial GR protein expression in WT embryos (n 10–12 embryos) at 24 hpf (I), 36 hpf (II), and 48 hpf (III). Fluorescent images (left) show GR protein distribution detected by specific antibody (polyclonal rabbit <t>antizebrafish</t> GR) binding, brightfield images (middle) display whole embryo shape, and negative control (Neg. Ctrl.) images (right) show specificity of fluorescence in embryo tissue.
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    FIG. 1. GR gene and protein expression in WT embryos. GR mRNA and protein exhibit dynamic spatial expression during early embryogenesis. A, Spatial GR mRNA abundance in WT embryos at 16-cell (I), 4-hpf (II), 6-hpf (III), 8-hpf (IV), 10-hpf (V), 12-hpf (VI), 24-hpf (VII), and-48 hpf (VIII) stages (n 10–15 embryos at each stage). Sense strand hybridizations at 24 hpf (IX) and 48 hpf (X) display probe specificity. B, Spatial GR protein expression in WT embryos (n 10–12 embryos) at 24 hpf (I), 36 hpf (II), and 48 hpf (III). Fluorescent images (left) show GR protein distribution detected by specific antibody (polyclonal rabbit <t>antizebrafish</t> GR) binding, brightfield images (middle) display whole embryo shape, and negative control (Neg. Ctrl.) images (right) show specificity of fluorescence in embryo tissue.
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    FIG. 1. GR gene and protein expression in WT embryos. GR mRNA and protein exhibit dynamic spatial expression during early embryogenesis. A, Spatial GR mRNA abundance in WT embryos at 16-cell (I), 4-hpf (II), 6-hpf (III), 8-hpf (IV), 10-hpf (V), 12-hpf (VI), 24-hpf (VII), and-48 hpf (VIII) stages (n 10–15 embryos at each stage). Sense strand hybridizations at 24 hpf (IX) and 48 hpf (X) display probe specificity. B, Spatial GR protein expression in WT embryos (n 10–12 embryos) at 24 hpf (I), 36 hpf (II), and 48 hpf (III). Fluorescent images (left) show GR protein distribution detected by specific antibody (polyclonal rabbit <t>antizebrafish</t> GR) binding, brightfield images (middle) display whole embryo shape, and negative control (Neg. Ctrl.) images (right) show specificity of fluorescence in embryo tissue.
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    Image Search Results


    FIG. 1. GR gene and protein expression in WT embryos. GR mRNA and protein exhibit dynamic spatial expression during early embryogenesis. A, Spatial GR mRNA abundance in WT embryos at 16-cell (I), 4-hpf (II), 6-hpf (III), 8-hpf (IV), 10-hpf (V), 12-hpf (VI), 24-hpf (VII), and-48 hpf (VIII) stages (n 10–15 embryos at each stage). Sense strand hybridizations at 24 hpf (IX) and 48 hpf (X) display probe specificity. B, Spatial GR protein expression in WT embryos (n 10–12 embryos) at 24 hpf (I), 36 hpf (II), and 48 hpf (III). Fluorescent images (left) show GR protein distribution detected by specific antibody (polyclonal rabbit antizebrafish GR) binding, brightfield images (middle) display whole embryo shape, and negative control (Neg. Ctrl.) images (right) show specificity of fluorescence in embryo tissue.

    Journal: Endocrinology

    Article Title: Glucocorticoid receptor signaling is essential for mesoderm formation and muscle development in zebrafish.

    doi: 10.1210/en.2011-1559

    Figure Lengend Snippet: FIG. 1. GR gene and protein expression in WT embryos. GR mRNA and protein exhibit dynamic spatial expression during early embryogenesis. A, Spatial GR mRNA abundance in WT embryos at 16-cell (I), 4-hpf (II), 6-hpf (III), 8-hpf (IV), 10-hpf (V), 12-hpf (VI), 24-hpf (VII), and-48 hpf (VIII) stages (n 10–15 embryos at each stage). Sense strand hybridizations at 24 hpf (IX) and 48 hpf (X) display probe specificity. B, Spatial GR protein expression in WT embryos (n 10–12 embryos) at 24 hpf (I), 36 hpf (II), and 48 hpf (III). Fluorescent images (left) show GR protein distribution detected by specific antibody (polyclonal rabbit antizebrafish GR) binding, brightfield images (middle) display whole embryo shape, and negative control (Neg. Ctrl.) images (right) show specificity of fluorescence in embryo tissue.

    Article Snippet: The proteins were transferred onto a nitrocellulose membrane and were probed with the rabbit antizebrafish GR primary antibody (1: 750 dilution) and goat antirabbit horseradish peroxidase-conjugated secondary antibody (1:3000 dilution; Bio-Rad, Hercules, CA) for 1 h each at room temperature with gentle rocking.

    Techniques: Expressing, Binding Assay, Negative Control, Fluorescence